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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Hepatol Res. 2014 Jun 4;45(3):305–314. doi: 10.1111/hepr.12352

Figure 6.

Figure 6

Amitriptyline (TCA) inhibits collagen expression and proliferation of activated hepatic stellate cells in vitro. (A) Treatment with 25μM TCA caused a marked reduction in collagen and alpha-smooth muscle actin (α-SMA) expression by activated HSCs, which was maintained despite stimulation with 5nM of transforming growth factor beta type one (TGF-β1) or 50 nM platelet-derived growth factor-beta, beta (PDGF-BB). *p<0.05 compared to untreated, TGF-β1, PDGF-BB alone groups. (B) Treatment with 25μM TCA caused a marked reduction in proliferation by activated HSCs as measured by BrdU incorporation. Stimulation of HSCs with 50nM of PDGF-BB markedly increased proliferation. Concomitant treatment with TCA completely abrogated this effect. Data are expressed as mean and SEM. *p<0.05 compared to untreated PDGF-BB alone groups; **p<0.05 compared to all other groups.