Table 2. Major altered genes categorized as transcription regulators, enzymes, transmembrane receptors and cytokines in THP-1 cells after L-92 treatment for 4 hr and 24 hr.
| Category | Entrez Gene Name | Symbol | Fold change |
|
| 0–4 hr | 4–24 hr | |||
| Early responding genes | > 4.0 | 4.0 > | ||
| Cytokine | ||||
| chemokine (C-C motif) ligand 4 | CCL4 | 58.29 | 2.02 | |
| chemokine (C-X-C motif) ligand 11 | CXCL11 | 27.97 | 2.28 | |
| chemokine (C-C motif) ligand 3 | CCL3 | 20.04 | 2.77 | |
| tumor necrosis factor | TNF | 14.57 | 1.15 | |
| interleukin 1 receptor antagonist | IL1RN | 5.11 | 1.95 | |
| Transmembrane receptor | ||||
| intercellular adhesion molecule 1 | ICAM1 | 10.01 | 2.33 | |
| toll-like receptor 7 | TLR7 | 6.63 | 0.32 | |
| Enzyme | ||||
| superoxide dismutase 2, mitochondrial | SOD2 | 10.04 | 2.50 | |
| heme oxygenase (decycling) 1 | HMOX1 | 4.26 | 2.21 | |
| Transcription regulator | ||||
| jun proto-oncogene | JUN | 4.99 | 2.72 | |
| nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 | NFKB1 | 5.18 | 2.21 | |
| nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) | NFKB2 | 6.10 | 1.85 | |
| human immunodeficiency virus type I enhancer binding protein 2 | HIVEP2 | 4.18 | 2.29 | |
| v-rel reticuloendotheliosis viral oncogene homolog B | RELB | 4.34 | 1.04 | |
| Slowly responding genes | 0–4 hr | 4–24 hr | ||
| Transmembrane receptor | 4.0 > | > 4.0 | ||
| interleukin 7 receptor | IL7R | 1.83 | 63.99 | |
| CD80 molecule | CD80 | 1.74 | 61.40 | |
| cytokine receptor-like factor 2 | CRLF2 | 2.02 | 52.00 | |
| CD86 molecule | CD86 | 0.86 | 30.10 | |
| CD5 molecule | CD5 | 1.10 | 16.20 | |
| interleukin 15 receptor, alpha | IL15RA | 2.06 | 4.37 | |
| major histocompatibility complex, class II, DQ alpha 1 | HLA-DQA1 | 0.94 | 5.93 | |
| interleukin 2 receptor, alpha | IL2RA | 1.00 | 4.98 | |
| colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) | CSF2RA | 0.85 | 4.15 | |
| Cytokine | ||||
| interleukin 12B (natural killer cell stimulatory factor 2, p40) | IL12B | 3.39 | 18.10 | |
| interleukin 6 (interferon, beta 2) | IL6 | 1.08 | 54.45 | |
| interleukin 23, alpha subunit p19 | IL23A | 1.29 | 28.01 | |
| secreted phosphoprotein 1 | SPP1 | 2.73 | 11.06 | |
| chemokine (C-C motif) ligand 22 | CCL22 | 1.07 | 17.16 | |
| chemokine (C-C motif) ligand 19 | CCL19 | 1.13 | 9.65 | |
| Transcription regulator | ||||
| early B-cell factor 1 | EBF1 | 1.12 | 41.89 | |
| signal transducer and activator of transcription 4 | STAT4 | 1.42 | 23.28 | |
| runt-related transcription factor 1; translocated to, 1 (cyclin D-related) | RUNX1T1 | 0.97 | 8.18 | |
| zinc finger protein, multitype 2 | ZFPM2 | 1.05 | 5.65 | |
| B-cell CLL/lymphoma 11A (zinc finger protein) | BCL11A | 0.85 | 6.92 | |
| growth factor independent 1 transcription repressor | GFI1 | –2.21 | –5.00 | |
| Enzyme | ||||
| prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) | PTGS2 | 2.58 | 14.29 | |
| adenosine deaminase | ADA | 1.07 | 9.62 | |
| sphingosine-1-phosphate lyase 1 | SGPL1 | 1.06 | 5.90 | |
| 3’-phosphoadenosine 5’-phosphosulfate synthase 2 | PAPSS2 | 1.05 | 5.47 | |
| fibronectin 1 | FN1 | 0.95 | 5.84 | |
| coagulation factor XIII, A1 polypeptide | F13A1 | –1.33 | –12.62 | |
| Constitutively responding genes | 0–4 hr | 4–24 hr | ||
| Cytokine | > 4.0 | > 4.0 | ||
| interleukin 1, beta | IL1B | 26.00 | 4.09 | |
| interleukin 8 | IL8 | 16.28 | 4.84 | |
| interleukin 1, alpha | IL1A | 4.32 | 16.48 | |
| Epstein-Barr virus induced 3 | EBI3 | 6.64 | 7.44 | |
| Enzyme | ||||
| indoleamine 2,3-dioxygenase 1 | IDO1 | 5.67 | 30.67 | |
Early responding genes were those altered at 4 hr; slowly responding genes were those altered at 24 hr.