Fig. 4.

Pma1 RNA and protein expression. (A) Wild type and L16 mutant cells were grown in SD medium at 30 °C. Total RNAs were isolated from cells at the log phase (OD₆₀₀ = 1.5) and subjected to Northern blotting analysis with a radiolabeled pma1⁺ probe. An ethidium bromide stained gel shows that total RNA was present as a loading control. (B) Wild type and L16 mutant cells were grown as described above, after which cell lysates were prepared. Pma1 protein expression was assessed by Western blotting using anti-Pma1 serum. Tubulin was used as a loading control.