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Cloned calves produced by nuclear transfer using cumulus cells under 4 different conditions: (a) cells removed from cumulus-oocyte complexes (COCs) after aspiration of ovarian follicles; (b) cells removed from COCs after in vitro maturation; (c) cells cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) for 3 days after some subculture; and (d) cells cultured in DMEM with 0.5% FBS for an additional 5 days.
