. 2014 Jun 6;60(5):336–341. doi: 10.1262/jrd.2013-116

Table 1. In vitro development of swamp buffalo cloned embryos after TSA treatment for 10 h.

Treatment	No. of replications	Fused (%)	Cultured	Cleaved (%)	No. (%)* embryos developed to
Treatment	No. of replications	Fused (%)	Cultured	Cleaved (%)	8-cells	Morula	Blastocyst
0 nM	11	120/140	120	96	60	33	22
0 nM	11	(85.1 ± 6.4)	120	(81.9 ±13.2)	(62.9 ± 15.0)	(34.8 ± 8.1)^b	(23.4 ± 6.0)^b
25 nM	12	117/143	117	91	58	38	26
25 nM	12	(81.6 ± 5.3)	117	(78.6 ± 19.1)	(65.4 ± 13.9)	(42.7 ± 9.6)^a	(30.1 ± 8.1)^a
50 nM	10	121/146	118	96	57	33	25
50 nM	10	(82.9 ± 2.2)	118	(84.1 ± 11.8)	(59.2 ± 11.4)	(37.8 ± 4.6)^b	(25.4 ± 4.0)^ab

* Percentages calculated from the number of cleaved embryos and that developed to each stage. Values are means ± SD. Different superscripts within a column indicate significant differences (P < 0.05; Duncan’s multiple range test).