Table 1. Composition of extenders used for semen cooling and freezing.

	C-Ext I	C-Ext II	F-Ext I	F-Ext II
Trizma base	3.025 g	3.025 g	3.025 g	3.025 g
Citric acid	1.7 g	1.7 g	1.7 g	1.7 g
Fructose	1.25 g	1.25 g	1.25 g	1.25 g
Streptomycin	1 mg/ml	1 mg/ml	1 mg/ml	1 mg/ml
Penicillin	1 mg/ml	1 mg/ml	1 mg/ml	1 mg/ml
Glycerol	3%	–	7%	10%
Equex STM Paste	–	–	1%	1%
Egg yolk	20%	20%	20%	20%
Milli-Q water	77 ml	80 ml	72 ml	69 ml
pH	6.76	6.72	6.70	6.76
Osmolarity	870 mOsm	335 mOsm	1570 mOsm	1978 mOsm

Cooling extenders (C-Ext I and II)/freezing extenders (F-Ext I and II).