Table 2. Mean percentages of total motility, progressive motility and viability in frozen-thawed semen samples at 30 min after thawing (experiment 1).
Cooling rate | Glycerol addition protocol | Total motility (%) |
Progressive motility (%) |
Sperm viability (%) |
Control | Fractionated | 61.5a | 44.9a | 64.5a |
Unfractionated | 66.4b | 50.5b | 67.7b | |
Rapid | Fractionated | 62.7ab | 46.0ab | 65.4ab |
Unfractionated | 64.7ab | 46.0ab | 66.3ab | |
SEM | 2.4 | 2.0 | 1.6 | |
Probability | Cooling rate | NS | NS | NS |
Glycerol addition protocol | 0.003 | 0.025 | 0.000 | |
Interaction | NS | NS | NS |
Data are the LSM ± SEM of five replicates per semen pool. a–b Different letters within the same column indicate significant differences (P<0.05) among the glycerol addition protocols.