. 2014 Jul 21;60(5):355–361. doi: 10.1262/jrd.2014-024

Table 3. Mean percentages of total motility, progressive motility and viability in frozen-thawed semen samples at 150 min after thawing (experiment 1).

Cooling rate	Glycerol addition protocol	Total motility (%)	Progressive motility (%)	Sperm viability (%)
Control	Fractionated	53.8^ab	39.3^ab	62.1^a
Control	Unfractionated	57.8^b	42.6^b	66.5^b
Rapid	Fractionated	52.2^a	37.4^a	61.7^a
Rapid	Unfractionated	56.5^ab	40.4^ab	64.1^ab
SEM		2.2	2.2	2.7

Probability	Cooling rate	NS	NS	NS
	Glycerol addition protocol	0.001	0.013	0.000
	Interaction	NS	NS	NS

Data are the LSM ± SEM of five replicates per semen pool. ^a–b Different letters within the same column indicates significant differences (P<0.05) among the glycerol addition protocols.