Table 3. Mean percentages of total motility, progressive motility and viability in frozen-thawed semen samples at 150 min after thawing (experiment 1).
Cooling rate | Glycerol addition protocol | Total motility (%) |
Progressive motility (%) |
Sperm viability (%) |
Control | Fractionated | 53.8ab | 39.3ab | 62.1a |
Unfractionated | 57.8b | 42.6b | 66.5b | |
Rapid | Fractionated | 52.2a | 37.4a | 61.7a |
Unfractionated | 56.5ab | 40.4ab | 64.1ab | |
SEM | 2.2 | 2.2 | 2.7 | |
Probability | Cooling rate | NS | NS | NS |
Glycerol addition protocol | 0.001 | 0.013 | 0.000 | |
Interaction | NS | NS | NS |
Data are the LSM ± SEM of five replicates per semen pool. a–b Different letters within the same column indicates significant differences (P<0.05) among the glycerol addition protocols.