. 2014 Jul 21;60(5):355–361. doi: 10.1262/jrd.2014-024

Table 4. Individual mean percentages of total motility, progressive motility and viability at 30 min after thawing in frozen-thawed semen samples frozen using different cooling rates and an unfractionated glycerol addition protocol (experiment 2).

Male	Cooling rate	Total motility (%)	Progressive motility (%)	Sperm viability (%)
1	Control	71.4^ab	57.7^a	73.2^a
1	Rapid	74.0^a	56.3^a	73.8^a
2	Control	64.4^abc	47.8^ab	71.0^a
2	Rapid	60.7^bc	45.5^ab	68.2^ab
3	Control	60.1^bc	49.9^ab	60.5^b
3	Rapid	63.7^abc	53.7^ab	61.0^b
4	Control	58.7^c	46.5^ab	65.3^b
4	Rapid	60.9^bc	43.9^b	65.5^b
SEM		3.7	4.4	2.3

Probability	Male	0.001	0.000	0.000
	Cooling rate	NS	NS	NS
	Interaction	NS	NS	NS

Data are the LSM ± SEM of five replicates per male. ^a–c Different letters within the same column indicates significant differences (P<0.05) among the males.