Table 4. Individual mean percentages of total motility, progressive motility and viability at 30 min after thawing in frozen-thawed semen samples frozen using different cooling rates and an unfractionated glycerol addition protocol (experiment 2).
Male | Cooling rate | Total motility (%) |
Progressive motility (%) |
Sperm viability (%) |
1 | Control | 71.4ab | 57.7a | 73.2a |
Rapid | 74.0a | 56.3a | 73.8a | |
2 | Control | 64.4abc | 47.8ab | 71.0a |
Rapid | 60.7bc | 45.5ab | 68.2ab | |
3 | Control | 60.1bc | 49.9ab | 60.5b |
Rapid | 63.7abc | 53.7ab | 61.0b | |
4 | Control | 58.7c | 46.5ab | 65.3b |
Rapid | 60.9bc | 43.9b | 65.5b | |
SEM | 3.7 | 4.4 | 2.3 | |
Probability | Male | 0.001 | 0.000 | 0.000 |
Cooling rate | NS | NS | NS | |
Interaction | NS | NS | NS |
Data are the LSM ± SEM of five replicates per male. a–c Different letters within the same column indicates significant differences (P<0.05) among the males.