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. 2014 Aug 4;60(5):388–394. doi: 10.1262/jrd.2014-004

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©2014 Society for Reproduction and Development

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 4. — Subcellular localization of p-p38 in matured porcine oocytes in the non-heat-shocked (39 C) and heat-shocked (41.5 C) groups. Green dots are p-p38 labeled by immunocytochemical staining. (A–E) Expressions of p-p38 in matured oocytes after heat shock for 0 h (A), 1 h (B), 2 h (C) or 4 h (D) and the p-p38 level in matured oocytes cultured at 39 C for 4 h (E). Panels A’–E’ are negative controls (without primary antibody), and insets are chromatin/chromosome (blue) structures stained by Hoechst 33342. Scale bar, 10 μm.