Fig. 1.

Deletions in the juxtamembrane domain of the FLT3 gene. (A) FLT3 ITD fragment analysis showing wild-type peak (330 bp), and smaller PCR amplification products (327 bp and 318 bp) which correspond to a 3-bp deletion in patient #1 and a 12-bp deletion in patient #2, respectively. (B) Results of Sanger sequencing confirming the deletions. The wild-type (WT) FLT3 sequence is in lowercase. The sequence of the deletion is boxed. The deletion mutation starting point is depicted by ▲(red) in the respective read directions, and the insertion is indicated by a blue underline. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)