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Rab11a binding is dependent on the integrity of the C terminus of rhodopsin. Pull-down experiments using a rab11a antibody show that wild-type rhodopsin can be pulled down by rab11a from retinal extracts but not Q344X or rhodopsin-EGFP. I, Input; C, control IgG antibody wash; Ab, rab11 polyclonal antibody eluate; IP, immunoprecipitation; IB, immunoblot.
