Figure 3.

hM4D-mediated inhibition of vHPC PV neurons reduced percentage PPI, startle amplitude, and spontaneous alternation, but did not affect social interaction. PV-Cre mice expressing hM4D in PV neurons of the vHPC were administered vehicle or CNO intraperitoneally. A, CNO treatment (5 mg/kg, n = 10) reduced percentage PPI compared with vehicle treatment (n = 10). *p < 0.05; **p < 0.01. B, Startle amplitude during pulse-alone (120 dB) trials was significantly reduced in CNO-treated compared with vehicle-treated mice. **p < 0.01. C, CNO-treated mice (5 mg/kg, n = 7) alternated significantly less than vehicle-treated mice (n = 8). **p = 0.01. Di, Dii, Percentage time spent in the stranger and empty chambers (i) and distance traveled during the social interaction test (ii) was not significantly different between vehicle and CNO treatment (5 mg/kg, n = 12). Data are presented as mean ± SEM.