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. 2014 Nov 5;34(45):14961–14972. doi: 10.1523/JNEUROSCI.2676-14.2014

Figure 2.

Figure 2.

The Wnt5-expressing DLC cells are a previously undescribed type of AL-associated neurons. 16 hAPF ALs from wnt5Gal4; UAS-mCD8::GFP animals stained with antibodies against CD8 to visualize the wnt5-expressing cells (green) and against either Elav (A), Acj6 (B), or Drifter (Dfr) (C; magenta). The developing 16 hAPF AL is highlighted with dotted lines. The bundle of GFP+ fibers terminating on the dorsolateral margin of the AL could be traced to a group of ∼20–30 cells (*) located ∼50 μm anterior and lateral to the AL in the deutocerebrum, which we called DLC cells (A1). The cells express the Elav protein in their nuclei indicating that they were neurons (A2). The cells did not express Acj6, or Dfr, although they are located close to clusters of Acj6- or Dfr-expressing PN cell bodies (B1, B2, C1, C2, arrowheads). These results, together with the observation that these cells did not extend dendrites into the AL or axons to the mushroom body suggested that they were not PNs, but were likely a novel type of AL-associated neurons. Scale bars, 10 μm.