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. 2014 Nov 5;34(45):14973–14983. doi: 10.1523/JNEUROSCI.1156-14.2014

Figure 5.

Figure 5.

The effects of genetic loss of Smad4 gene expression on OPC migration. A, A′, To remove the Smad4 gene expression in the OPC lineage, we used Sox10-Cre to conditionally inhibit Smad4. At E15.5 when OPCs migrate into the cortex we counted OPCs labeled with Pdgfrα and Olig2 in the cortex above the dotted line. A graph depicts the quantitative results from four litters (A′; n = 4). B, B′, A day later at E16.5, we counted OPCs in the cortex (pallial) and subcortical boxed regions (subpallial). A graph depicts the quantitative results from four litters (n = 4; B′). C, C′, A day before the mutant embryos die, we stained the cortex with Pdgfrα to show the OPCs at E17.5. A plot was made from data collected from three litters at E17.5 (n = 4). D, E17.5 forebrains were stained for Pdgfrα to show the heterotopic OPCs in the two main germinal areas, SVZ, and third ventricle (3rd V) in the mutant embryos. Scale bars: 100 μm. Error bars depict SEM.