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. 2014 Oct 27;35(11):1351–1363. doi: 10.1038/aps.2014.85

Figure 6.

Figure 6

Effects of DS-201 on the subcellular localization of BKCa channel α subunits in HEK293 cells. (A) Original FCM recording showing that 80 μmol/L DS-201 increased the percentage of GFP-positive (the first plus the fourth quadrant) and Flag-positive cells (the first quadrant) in HEK293 cells expressing the α subunit. Untransfected HEK293 cells served as negative controls. (B) Statistical results from (A). GFP+, GFP-positive cells. Flag+, Flag-positive cells (n=8). (C) The statistical results showing the effects of 80 μmol/L DS-201 on the MFIFlag/MFIGFP ratio, representing the membranous level of the α subunit (n=8). (D) Confocal images showing the membranous and intracellular distributions of hSlo. GFP (green) and anti-Flag (red) represent the global and membranous hSlo, respectively. Note that DS-201-treated cells show increased membranous hSlo, compared with control cells. Scale bar=50 μm. bP<0.05 vs control.