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Constructions of the recombinant eukaryotic expression plasmids. (A) Electrophoretogram of the PCR products. M, low molecular weight DNA markers; 1, IL-6Rα gene; 2, IL-6 gene. The arrows separately indicate the theoretical molecular weights of the IL-6Rα and IL-6 genes (similarly hereinafter). (B) Electrophoretogram of the recombinant plasmids identified by the restriction enzyme digestion. M, high molecular weight DNA markers; 1, plasmid pABhFc-IL6; 2, plasmid pTaglite-SNAP-IL6R.
