Skip to main content
. 2014 Oct 27;35(11):1453–1462. doi: 10.1038/aps.2014.90

Figure 2.

Figure 2

Identification and quantification of the expression level of IL-6R. (A) Indirect immunofluorescence assay for identification. The images, from top to bottom, represent the IL-6R protein, nucleus and merged images with the IL-6R protein and nucleus. IL-6R was detected by Alexa Fluor® 488-labeled immunostaining (Green. Exposure time, 0.08 s); the nuclei were stained by DAPI (Blue. Exposure time, 0.02 s). Scale bar, 100 μm. Camera, ORCA-R2 in the Cellomics arrayscan VTI HCS reader system. (B) The Western blot assay for the quantitative analysis. The bands for IL-6R were normalized to β-actin. The data represent the mean±SEM from three separate experiments. cP<0.01, significant compared with the control group (untreated) and the control plasmid pTaglite-SNAP group (blank load).