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. 2014 Oct 27;35(11):1453–1462. doi: 10.1038/aps.2014.90

Figure 3.

Figure 3

Expression and functional identification of rhIL-6 obtained from the transfected HEK293A cells. (A) SDS-PAGE analysis for the secretion of rhIL-6. M, protein markers; 1, non-reductive form of rhIL-6; 2, reductive form of rhIL-6 (loading buffer containing 5% β-mercaptoethanol). (B) The 7TD1 cell number changed over time in the mediums with different concentrations of rhIL-6. The data represent the mean±SEM from five separate experiments. (C) The 7TD1 cell viabilities declined, accompanied by multiple proportional dilutions of rhIL-6 (the initial concentration of rhIL-6 was 20 ng/mL). The data represent the mean±SEM from four separate experiments. (D) The Western blot assay for the quantitative analysis of protein phosphorylation of the JAK/STAT3 signal pathway mediated by rhIL-6 in the U937 cells. The bands for p-STAT3 were normalized to total-STAT3. The data represent the mean±SEM from four separate experiments. cP<0.01, significant compared with the untreated control.