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. 2014 Oct 27;35(11):1453–1462. doi: 10.1038/aps.2014.90

Figure 4.

Figure 4

Establishment of a screening assay based on the cellular ELISA. (A) The binding capacity of rhIL-6 coupled with the Fc fragment and a secondary antibody (anti-human IgG goat mAb, HRP conjugate). OD492–630 refers to the difference between the absorbance at a wavelength of 492 nm and the absorbance at a wavelength of 630 nm (similarly hereinafter). The data represent the mean±SEM from three separate experiments. (B) RhIL-6 was able to bind to IL-6R expressed at the HEK293A cell surface in a dose-dependent manner. The data represent the mean±SEM from five separate experiments. cP<0.01, significant compared with the PBS control group. (C and D) Effects of different temperature states and different blocking buffers on the binding capacity of rhIL-6 and its membrane-bound receptor.