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. 2014 Nov 5;4:6926. doi: 10.1038/srep06926

Figure 5. Site-specific insertion of HindIII site mediated by TALENs.

Figure 5

(A) Schematic of the DJ-1 locus with the ssODN sequence used to introduce the HindIII sequence (underlined) into the genome in vivo. (B) One of the colonies (DS21) could be cut by the HindIII restriction enzyme. The arrow shows the resulting fragments cut by HindIII. (C) DNA sequences of the DS21 colony at the target site. The HindIII site is shown by bold uppercase letters. There is a different base before the HindIII site, as shown in lowercase letters. DS21-WT represents sequence of the mixed wild type cells in DS21 colony.