Figure 5. The uptake ability of fluorescent nanodiamonds in dissociated neurons by flow cytometry analysis.

(A) Dissociated mouse cortical neurons treated with 0, 50, or 250 μg/mL FND for 3 days. FNDs were excited by with the 488 nm wavelength light, and the emitted light was collect in >650 nm wavelength range. (B) The fluorescence intensity of FND was quantified from a minimum of 10,000 cells using CellQuest software. Data were collected from 3 independent experiments, and the bar graph is expressed as mean ± SEM.