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. 2014 Nov 5;4:6919. doi: 10.1038/srep06919

Figure 7. Fluorescent nanodiamonds reduced neurite outgrowth in a dosage-dependent manner in dissociated hippocampal neurons.

Figure 7

(A) Images of dissociated hippocampal neurons treated with various concentrations of FNDs 4 hours after seeding and incubated for 3 days in vitro. Images on the top row show β-III-tubulin staining (inverted to enhance visibility), merged images on the bottom row show FND (red) and β-III-tubulin staining (green). Scale bars represent 100 μm. (B) Quantification of total neurite length per neuron in FND treated hippocampal neurons. All quantification data were obtained from 3 independent repeats and normalized to the group without FND. * p < 0.05, ** p < 0.01, *** p < 0.001 one-way ANOVA followed by Dunnett's post-hoc analysis against the control group (0 μg/mL FND). The bar graph is expressed as mean ± SEM.