Figure 3. Major non-invasive endothelial function tests.
After 5 minutes forearm occlusion, a pneumatic pressure cuff is rapidly deflated to induce reactive hyperemia. Maximal response occurs in the 60- to 90-second post-deflation interval. To detect reactive hyperemia, brachial artery diameter change is used in FMD and augmentation of fingertip pulse amplitude is used in RH-PAT.
FMD: flow mediated vasodilatation, and RH-PAT: reactive hyperemia-peripheral arterial tonometry.