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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mech Dev. 2013 Dec 14;131:137–149. doi: 10.1016/j.mod.2013.12.002

Fig. 3.

Fig. 3

Sonic, Indian and Desert HH are able to activate a Gli response in co-plating cultures. (A) Luciferase assays were performed on Light2 cells co-plated with 293T cells transfected with full length HH constructs for 48 h in media containing 0.5% FBS. Luciferase activity is shown relative to mock transfected control. (B) Gli1 protein levels were determined by Western blot of cell lysates from co-plating cultures. (C) qPCR for Gli1 and Ptch1 mRNA was performed on RNA extracted from TM3 cells co-plated for 48 h with 293T cells transfected with full length HH constructs in medium with 0.5% FBS. mRNA expression levels are shown as a % of expression in SHH transfected co-culture.