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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mech Dev. 2013 Dec 14;131:137–149. doi: 10.1016/j.mod.2013.12.002

Fig. 5.

Fig. 5

Prostate cancer cell lines express variable amounts of HH ligand capable of inducing a juxtacrine signaling response in coplating cultures. (A) RNA obtained from LnCAP prostate cancer cells grown in normal growth conditions, and also for 2 weeks in androgen deprived (AD) conditions to confluence or to sub-confluent levels was used in qPCR to determine endogenous SHH, DHH (B) and IHH (C) mRNA levels, as well as Gli1, Gli2 and Gli3 mRNA expression (D). (E) Luciferase assays were performed on Light2 cells co-plated for 48 h in androgen deprived media with LnCAP cells previously grown for 2 weeks in AD media. Luciferase activity is shown relative to Light2 cells plated alone.