Table 1.

Imaging Method Advantages and Contributions

	Cryo-EM-Tomography	Structured Illumination Microscopy	Spinning Disk Confocal Microscopy
Spatial resolution (x and y)	1 nm	100 nm	200 nm
Spatial resolution (z)	2–8 nm	350 nm	500 nm
Temporal resolution	none	seconds	milliseconds
Main application in cell biology	Subnanometer 3D spatial architecture of the entire cellular content	3D structure analysis of selected macromolecular complexes and organelles in cells	3D protein and membrane dynamics in live cells
General advantages for applications in cell biology	Subnanometer spatial architecture 3D structural information Visualization of both protein and lipid structures	Subdiffraction resolution 3D imaging Fluorescent labeling of specific molecules	High-speed image acquisition Quantitative image analysis 3D imaging Fluorescent labeling of specific molecules
Insights into cytokinetic abscission	Abscission site location Description of helical cortical filaments at abscission site Microtubule organization and stability	Cortical ring organization of ESCRT proteins at the dark zone Localization of ESCRT-III proteins to the abscission site late in cytokinesis	Abscission site location Abscission timing Spatio-temporal dynamics of ESCRT proteins during late cytokinesis Microtubule and actin dynamics during cytokinesis Membrane dynamics at the intercellular bridge