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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Biomaterials. 2014 Jul 12;35(30):8635–8648. doi: 10.1016/j.biomaterials.2014.06.040

Figure 6. Effect of PLGA-CUR NPs on the AR and β-catenin.

Figure 6

(A) PLGA-CUR NPs lowers the AR expression in C4-2 cells. C4-2 cells were treated with 20 μM curcumin or equivalent PLGA-CUR NPs, or respective control (DMSO or PLGA-NPs) for 24 hrs, immunostained for membrane marker (green), AR (red) and counter-stained with DAPI (blue). Curcumin and PLGA-CUR NPs treated cells showed significantly lower AR staining compared to control C4-2 cells. (B) PLGA-CUR NPs treatment improves the cellular localization of β-catenin and PKD1. C4-2 cells were treated with 20 μM curcumin or equivalent PLGA-CUR NPs, or respective control (DMSO or PLGA-NPs) for 1 hr, immunostained for PKD1 (green), β-catenin (red) and counter-stained with DAPI (blue). CUR and PLGA-CUR NPs treated cells exhibited higher membrane β-catenin staining and localized with PKD1, compared to control C4-2 cells. Original Magnifications 600X.