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. 2014 Nov 5;5:603. doi: 10.3389/fpls.2014.00603

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Copyright © 2014 Schwinn, Boase, Bradley, Lewis, Deroles, Martin and Davies.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of transcript abundance for the anthocyanin biosynthesis genes CHS and ANS in 35S:ROS1 and non-transgenic lisianthus plants. (A) RNA samples (10 μg total RNA) from Stage 3 petals of three 35S:ROS1 transgenic lines (54-111, 54-14, and 54-11) and a non-transgenic (54) line. (B) RNA samples (20 μg of total RNA) from three petal stages (Stage 1,3, and 5), sepal, leaf of transgenic line 54-14. Northern RNA hybridisation was conducted against radiolabelled CHS, ANS and rRNA cDNA probes.