Figure 5.

Multiple sequence alignment of the sole ATP sulfurylase domain from different algal species showing cysteine residues (highlighted in yellow) and their conservation among species. A representative for each group of algal ATPS (see Figure 3) was chosen: Tetraselmis suecica was selected for green algae, Phaeodactylum tricornutum for diatoms and haptophytes (both the enzyme with the sole ATPS function and the ATPS domain in the APK-ATPS enzymes are shown), Cyanidioschyzon merolae for red algae (one plastidial and one cytosolic ATPS are present in this species, both constituted by the sole ATPS domain), Amphidinium klebsii for dinoflagellates (both the monofunctional ATPS and the ATPS domain of the APR-ATPS enzyme are shown), Synechococcus sp. strain WH7803 for marine cyanobacteria and Synechocystis sp. strain PCC6803 for freshwater cyanobacteria. The cysteine residues included in a red rectangle are present, and their position is conserved, in all the ATPS sequences belonging to the same group (as described in Figure 3). Alignments were performed using MUSCLE (http://www.ebi.ac.uk/Tools/msa/muscle/) and results exported and edited with GenDoc (http://www.nrbsc.org/gfx/genedoc/).