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. 2014 Sep 17;124(10):4642–4656. doi: 10.1172/JCI74084

Figure 3. DC accumulation of isoketals in hypertension.

Figure 3

(A) Intracellular staining of isoketals using Alexa Fluor 488–tagged D11 in CD11b+/CD11c–, (B) CD11b+/CD11c+, and (C) CD11b–/CD11c+ cells from sham and angiotensin II–infused mice with or without 2-HOBA treatment. Mean data are shown in the bar graphs (n = 6, *P < 0.05, **P < 0.01, ***P < 0.001). (D) Analysis of isoketals in various DC subtypes from sham or angiotensin II–infused mice (shown are representative data of 6 mice per group). (E) Stable isotope dilution multiple reaction monitoring mass spectrometry analysis of isoketal-lysine-lactam adduct in DCs. Representative LC/MS chromatographs from pooled samples for each group are shown. The top row shows multiple reaction monitoring chromatographs for isoketal-lysine-lactam (IsoK-Lys) in sample, while the bottom row shows multiple reaction monitoring chromatograph for [13C615N2] internal standard for same samples.