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. 2014 Sep 17;124(10):4642–4656. doi: 10.1172/JCI74084

Figure 8. Effect of isoketal protein modification on DC immunogenicity.

Figure 8

(A) MHCI was immunoprecipitated from DCs of sham and angiotensin II–treated mice, and Western blot using D11 antibody was performed. Mouse kidney homogenates (100 μg total protein) were exposed to 100 μmol/liter of isoketal, hydroxynonenal (HNE), MDA, or methylglyoxal (MGO) for 30 minutes. One million DCs were then pulsed with these modified proteins for 1 hour, washed, and exposed to T cells prelabeled with CFSE at a ratio of 1 DC to 10 T cells for 7 days. (B) Effect of DCs pulsed with lipid-modified proteins on CD8+ T cells. (C) Effect of DCs pulsed with lipid-modified proteins on CD4+ T cells. The left and middle panels of B and C show proliferation of T cells that were obtained from sham or angiotensin II–treated mice, respectively. The right panels of B and C show the effects of the lipid adducts directly added to DCs. (D) Proliferation of memory and naive T cells from angiotensin II–treated mice in response to DCs pulsed with isoketal-adducted proteins.