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. 2014 Nov 1;30(9):757–770. doi: 10.1089/jop.2014.0020

FIG. 7.

FIG. 7.

Immunohistochemical and ELISA analyses of the neurotrophic factors BDNF, CNTF, and bFGF in the retinas of non-c-Myc iPSC–transplanted and control rats. Immunohistochemical staining against (A) bFGF, (B) CNTF, and (C) BDNF was performed 14 days after transplantation. Specimens stained with IgG isotype antibody as the primary antibody were used as negative controls. bFGF immunoreactivity was significantly elevated in the INL and GCL of non-c-Myc iPSC–treated retinas (N=3 in each group). The expression of (D) bFGF, (E) CNTF, and (F) BDNF in each treatment group was quantified by ELISA analysis at days 3, 7, 14, and 21. The amount of bFGF expression was significantly higher in non-c-Myc iPSC–treated retinas than in the normal, MEF-treated, and PQ-injected retinas at 7, 14, and 21 days after transplantation (N=5 in each group). Bars show the mean±SEM. The scale bar represents 50 μm. *P<0.05 compared with the “PQ” and “PQ+MEF” groups; #P<0.05 compared with the “normal” group; by 2-way ANOVA. BDNF, brain-derived neurotrophic factor; bFGF, basic fibroblast growth factor; CNTF, ciliary neurotrophic factor.