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. 2014 Nov 5;5:561. doi: 10.3389/fimmu.2014.00561

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Copyright © 2014 Tanaka, Ishima, Enoki, Kimachi, Shirai, Watanabe, Chuang, Maruyama and Otagiri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental protocol for the effective evaluation of HSA–Trx, Trx, or Tamiflu^® on influenza-induced ALI model mice. Either HSA–Trx or Trx was administrated intravenously (1.75 nmol/mouse) at 4 and 6 days after virus infection. Tamiflu^® was administrated intraperitoneally (10 mg/kg/day, 2 times/day) for a total of 4 days, starting 2 days after the virus infection. The efficacy of therapeutic agents was evaluated with mice sacrificed at 8 days after virus infection.