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. Author manuscript; available in PMC: 2015 May 30.
Published in final edited form as: J Proteomics. 2014 Apr 5;103:178–193. doi: 10.1016/j.jprot.2014.03.037

Figure 4. Anti-caveolin 1 labels hair cells and auditory neurons in chick cochlea.

Figure 4

(A) A radial section from the distal (low-frequency) portion of the chick basilar papilla is shown to illustrate the position of hair cells (HC) and supporting cells (SC) of the sensory epithelium, neighboring homogene cells (HG), and the tectorial membrane (TM). (B) Anti-caveolin 1 brightly labeled the basolateral portion of the hair cells. As is often the case, the tectorial membrane was non-specifically reactive with the secondary antibody. Supporting cells were not immunoreactive. (C) Primary auditory neurons were specifically labeled by anti-caveolin 1. (D1-4) Isolated hair cells were diffusely labeled by anti-caveolin 1, with approximately 50% of the cells also displaying punctate basolateral spots (among 20 imaged hair cells). Hair bundles (all pointing upward) were not immunoreactive. Scale bars, A-C = 10 μm, D = 20 μm.