Figure 7. The separation of DRM and non-DRM membrane fractions is dependent on TX-100 concentration.
Western blots and dot blots of fractionated whole cochlear lysates are shown following treatment with 1%, 0.5%, or 0.25% TX-100. Western blots were probed with pan-caveolin (Cav) and transferrin receptor (TfR) antibodies to identify DRM and non-DRM fractions, respectively. Dot blots were probed with HRP-conjugated CTX to identify GM1-enriched fractions.