Figure 2.

Illustration of tensioned synoviocyte bioscaffold synthesis: 1) the hyperconfluent cell sheet (“HCS”) is moved to the cell culture well using a pipette tip; 2) the wire hoop is placed over the HCS; 3) while holding the wire twist with a forceps, the HCS is doubled over one end of the wire hoop; 4) the ends of the doubled over HCS are grasped with forceps and tension is applied perpendicular to the wire hoop; 5) the HCS is wrapped over the opposite side of the wire hoop; 6) the triple wrap is finished, creating a tensioned synoviocyte bioscaffold with six cell layers.