Figure 7. Western blot probed with an anti-YFP antibodies.

The extracts obtained from identical amounts of either wild type (WT) or AC3362-YFP bearing cell walls by treatment with a hot SDS solution (SDS) or with an ammonium fluoride solution at pH 4–5 (AF) were loaded. In lane ‘ctrl.’ 100 ng recombinant Sil3-GFP (isolated from E. coli) was loaded.