Table 2. Kinetic constants for BaL and L499M BaL with ABTS and 2,6-DMP at acidic pH values.

		BaL		L499M BaL
Substrate	pH	K m (M)	V max (Umg1)	K m (M)	V max (Umg1)
ABTS	3.0	5.3 0.7	160.4 4.2	8.8 0.5	82.5 1.2
	4.0	14.0 1.0	126.9 2.6	19.3 1.0	74.2 1.1
	5.0	45.1 1.1	81.5 0.6	84 4	61.6 1.0
	6.0	148 4	41.8 0.5	500 19	40.4 0.6
2,6-DMP	3.0	41.8 2.7	52.4 1.0	420 18	27.1 0.4
	4.0	8.3 0.7	37.4 0.7	80.8 2.7	23.6 0.2
	5.0	2.2 0.3	25.0 0.4	35.1 1.4	23.0 0.2
	6.0	1.8 0.2	17.8 0.2	34.7 1.1	22.8 0.2