Figure 3.
Purification of heterododecamers. The peaks are represented using the absorbance (A 280 nm) as a function of the conductivity (mS cm−1). (a) Ion-exchange chromatography (Resource Q) of the total sample after the re-oligomerization process. The shaded areas correspond to the fractions grouped together for successive Mono Q purification. (b) Ion-exchange chromatography (Mono Q) of the two major peaks after the first Resource Q step. The shaded areas correspond to the fractions grouped together for size-exclusion chromatography on a Superose 6 column (Supplementary Fig. S10).