Table 1. Inflammasome-related differential gene expression in THP-1 macrophages in response to T. cruzi infection (± ATP) in comparison to normal controls.

Tc vs control at 3h			Tc + ATP vs control at 3 h			Tc + ATP vs Tc at 3 h
Gene name	ddCt log ratio	p value	Gene name	ddCt log ratio	p value	Gene name	ddCt log ratio	p value
CXCL1	−4.10	0.000	CXCL1	−4.48	0.000	ACTB	−2.41	0.002
TNF	−2.86	0.000	TNF	−3.68	0.000	CXCL2	−2.21	0.001
NFKBIA	−2.46	0.000	CXCL2	−5.36	0.000	PTGS2	−4.73	0.020
CXCL2	−3.14	0.000	NFKBIA	−3.24	0.000	TNF	−0.82	0.028
CIITA	3.07	0.001	TXNIP	2.46	0.000	CIITA	−1.68	0.037
BCL2	0.95	0.005	ACTB	−2.79	0.001	NLRP3	−0.65	0.051
NFKB1	−1.03	0.007	RIPK2	−1.99	0.001
RIPK2	−1.43	0.008	NFKB1	−1.25	0.002
TXNIP	1.56	0.007	MAPK3	1.42	0.006
MAPK1	0.79	0.010	PSTPIP1	1.11	0.006
PSTPIP1	1.03	0.010	NLRP3	−0.96	0.007
B2M	1.44	0.023	BCL2	0.86	0.009
SUGT1	0.86	0.026	SUGT1	1.03	0.010
TAB2	0.66	0.032	PYCARD	1.10	0.015
CCL5	−1.12	0.042	IL1B	−1.75	0.018
PEA15	0.57	0.043	RPL13A	0.78	0.025
PYCARD	0.86	0.049	IRF1	0.78	0.034
CCL2	−2.92	0.053	CCL2	−2.92	0.053
RPL13A	0.65	0.056	CCL5	−1.06	0.053

The 96-well RT Profiler Human Inflammasome PCR Arrays (SA Biosciences/Qiagen) were probed in triplicate with cDNA from THP-1 macrophages infected with T. cruzi (Tc) for 3 h or 18 h (with or without ATP) as described in Materials and Methods. The Ct values from qPCR data were analyzed by using open source HTqPCR v.1.7 software package (v.2.13). All array data were normalized by Quantile method and filtered to exclude genes that exhibited Ct values>35. The relative expression level of each target gene in treated cells was calculated using the formula, fold change  = 2-ΔΔCt, where ΔCt represents the Ct (sample) - Ct (control). LimmaCt in HT-qPCR package was employed for contrast analysis of all the groups included in experiment and identification of genes that were overall differentially expressed (p<0.05).