Table 2. Ingenuity iReport analysis of inflammasome-related datasets in THP-1 macrophages infected by T. cruzi for 3 h or 18 h (± ATP).

Pathways	Gene name	FoCus mols	P value
T. cruzi infection of THP-1 mφs vs control (3h)
Top biological and molecular functions
1) Inflammatory responses/infection	↓B2M, ↓BCL2, ↑CCL2, ↑CCL5, ↓CIITA, ↑CXCL1, ↑CXCL2, ↑NFKB1, ↑NFKB1A, ↓PYCARD, ↑RIPK2,↑TNF, ↓TXNIP	13	5.91E-12–2.49E-04
1a. Recruitment of neutrophils/phagoCytes	↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↓PYCARD, ↑RIPK2, ↑TNF	7	2.77E-10
1b. Activation of leukoCyte/lymphoCytes	↓BCL2, ↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↑NFKB1, ↑NFKB1A, ↓PYCARD, ↑TNF	9	1.89E-07
2) Cell death of immune cells (decreased)	↓B2M, ↓BCL2, ↑CCL2, ↑CCL5, ↓CIITA, ↑CXCL1, ↑CXCL2, ↓MAPK1, ↑NFKB1, ↑NFKB1A, ↓PEA15, ↓PYCARD, ↑RIPK2, ↓TAB2, ↑TNF, ↓TXNIP	16	4.50E-10
2a. Macrophage cell death (decreased)	↓BCL2, ↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↑NFKB1, ↑NFKB1A, ↓PYCARD, ↑TNF	9	3.78E-13
Toxicity analysis
Decreases MPT, mitoChondrial swelling	↓B2M, ↓BCL2, ↑NFKB1, ↓PYCARD, ↑TNF	5	7.27E-08
T. cruzi infection of THP-1 mφs vs control (3 h + ATP)
Top biological and molecular functions
1) Inflammatory responses/infection	↓BCL2, ↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↓IL1B, ↓IRF1,↑NFKB1, ↑NFKB1A, ↓NLRP3, ↑PTGS2, ↓PYCARD, ↑RIPK2,↑TNF, ↓TXNIP	16	7.54E-14–1.79E-05
1a. Recruitment/migration of phagoCytes/neutrophils	↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↓IL1B, ↑MAPK3, ↑NLRP3, ↓PYCARD, ↑RIPK2, ↑TNF	9	7.69E-09
1b. Activation of lymphoCyte/leukoCytes	↓BCL2, ↑CCL2, ↑CCL5, ↑CXCL1, ↓IL1B, ↓IRF1, ↑NFKB1, ↑PTGS2, ↓PYCARD, ↑RIPK2 ↑TNF, ↓TXNIP	12	3.44E-12
2) Cell death of immune cells/necrosis (decreased)	↓BCL2, ↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↓IL1B, ↓IRF1, ↑MAPK3, ↑NFKB1, ↑NFKB1A, ↑NLRP3, ↑PTGS2, ↓PYCARD, ↑RIPK2, ↑TNF, ↓TXNIP	16	9.06E-17–1.99E-05
2a. Cell death of phagoCytes, myeloid cells (decreased)	↓BCL2, ↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↓IL1B, ↓IRF1, ↑NFKB1, ↑NFKB1A, ↓PYCARD, ↑TNF	11	9.06E-17
Toxicity analysis
Decreased MPT, mitoChondrial swelling	↓B2M, ↓BCL2, ↑NFKB1, ↓PYCARD, ↑TNF	5	7.27E-08
T. cruzi infection of THP-1 mφs vs control (18 h)
Top biological and molecular functions
1) Cell death of immune cells (decreased)	↓ACTB, ↓CASP4, ↑CCL2, ↑CCL5, ↑CFLAR, ↓CTSB, ↑CXCL1, ↑CXCL2, ↓GAPDH, ↓HSP90AA1, ↓HSP90B1, ↑ IL1B, ↑IRAK1, ↓MAP3K7, ↓MAPK1, ↓MAPK3, ↓NAIP, ↑P2RX7, ↓PEA15, ↓PYCARD, ↓TAB2, ↑TNF, ↓TNFS14, ↓TRAF6	24	1.48E-12–2.60E-04
1a.Cell death of myeloid/phagoCytes	↓CASP4, ↑CCL2, ↑CCL5, ↑CFLAR, ↑CXCL1, ↑CXCL2, ↑ IL1B, ↑P2RX7, ↓PYCARD, ↑TNF	10	1.72E-12
2) Inflammation/infectious disease	↓CASP4, ↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↓HSP90AA1, ↑ IL1B, ↓MAPK1, ↓PANX1, ↑P2RX7, ↓PYCARD, ↑TNF, ↓TNFS14, ↓TRAF6	14	1.08E-06
2a. Activation of leukoCyte/lymphoCytes	↑CCL2, ↑CCL5, ↑CXCL1, ↓HSP90B1, ↓IL1B, ↓MAP3K7, ↓PYCARD, ↑TNF, ↓TNFS14	9	2.95E-07
2b. Migration of phagoCytes/neutrophils	↑CCL2, ↑CCL5, ↓CTSB,↑CXCL1, ↑CXCL2, ↑IL1B, ↑P2RX7, ↑TNF	8	2. 07E-07
Toxicity analysis
Gene regulation by PPARα	↓HSP90AA1, ↑IL1B, ↓MAP3K7, ↓MAPK1, ↓MAPK3, ↑TNF, ↓TRAF	7	4.16E-10
T. cruzi infection of THP-1 mφs vs control (18 h + ATP)
Top biological and molecular functions
1) Cell death (decreased)	↓ACTB, ↓B2M, ↓CASP4, ↑CCL2, ↑CCL5, ↓CHUK, ↓CTSB,↑CXCL1, ↑CXCL2,↓GAPDH, ↓HSP90AA1, ↓HS90B1, ↑IRAK1, ↑IRF1, ↓MAP3K7, ↓MAPK1 ↓MAPK12, ↑NFKB1A, ↑P2RX7, ↓PEA15, ↑PTGS2, ↓PYCARD, ↓TAB2, ↑TNF, ↓TNFS14, ↓TRAF6	26	6.53E-12–4.09E-04
1a.Cell death of myeloid/phagoCytes	↓CASP4, ↑CCL2, ↑CCL5, ↑CFLAR, ↑CXCL1, ↑CXCL2, ↑ IL1B, ↑P2RX7, ↓PYCARD, ↑TNF	10	1.48E-12
2) Inflammation/Infectious disease	↓ACTB, ↓B2M, ↓CASP4, ↑CCL2, ↑CCL5,↓CHUK, ↓CTSB, ↑CXCL1, ↑CXCL2, ↓HSP90B1, ↑IRAK1 ↓MAP3K7, ↑NFKB1A, ↑PTGS2, ↑P2RX7, ↓PYCARD, ↓TIRAP, ↑TNF, ↓TNFS14, ↓TRAF6	21	3.33E-10–4.43E-04
2a. Activation of leukoCyte/lymphoCytes	↓B2M, ↑CCL2, ↑CCL5, ↑CXCL1, ↑CXCL2, ↓MAP3K7, ↑TNF	7	9.31E-06
2b. Migration of phagoCytes/neutrophils	↑CCL2, ↑CCL5, ↓CHUK, ↓CTSB, ↑CXCL1, ↑TNF ↑CXCL2, ↑NFKB1A, ↑P2RX7, ↑PTGS2, ↓TIRAP	11	2.43E-04
Toxicity analysis
Gene regulation by PPARα	↓CHUK, ↓HSP90AA1, ↓MAP3K7, ↓MAPK1, ↓MAP3K7, ↑NFKB1A, ↑PTGS2, ↑TNF, TRAF6	9	2.11E-11

All differentially expressed proteins identified in THP-1 macrophages infected with T. cruzi for 3 or 18 h (±ATP) (listed in Table 1) were uploaded into Ingenuity Pathway Analysis (IPA) to interpret datasets in the context of biological proCesses and function, and pathway and molecular networks. Presented are the top networks with a p value <0.01 to which maximal number of the differentially expressed proteins identified in chagasic plasma (bolded letters) were assoCiated with. FoCus molecules are the number of differentially expressed plasma proteins assoCiated with an individual network.