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. Author manuscript; available in PMC: 2014 Nov 5.
Published in final edited form as: Cytometry A. 2009 Jan;75(1):75–82. doi: 10.1002/cyto.a.20691

Figure 1.

Figure 1

Detection of SP cells in porcine bone marrow. Porcine bone marrow cells were stained with 5 μM of Hoechst 33342 for 120 min at 37°C with or without 50 μM verapamil. SP cells analysis was performed on BD Bioscience LSRΠ with UV laser. Gating was based on live (negative for PI), singlet (based on light scatter) and CD45 negative cells (A). Side population cells (EPCs) were clearly detected after Hoechst 33342 staining, and gated side population cells (EPCs) account for 0.51% of the CD45 negative cells (B). In the presence of verapamil, gated side population cells almost completely disappeared (C).