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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: Radiat Res. 2014 Sep 17;182(4):420–429. doi: 10.1667/RR13726.1

FIG. 5.

FIG. 5

Downregulation of G6PD, a downstream target of FOXM1 sensitizes quiescent HNSCC to radiation. Panel A: Glucose consumption rate in control and 8 Gy irradiated quiescent Cal27 cells was measured at 48 h postirradiation. Panel B: A Q-RT-PCR assay was used to measure G6PD mRNA expression in control and 8 Gy irradiated quiescent Cal27 cells at 48 h postirradiation. Asterisk represents statistical significance compared to unirradiated quiescent Cal27 cells. n = 3; P < 0.05. Panel C: mRNA and protein expression of G6PD in shFOXM1 Cal27 cells was measured by using a Q-RT-PCR and Western blotting assays. Asterisks indicate statistical significance compared to noninduced shFOXM1 Cal27 cells. n =3; P < 0.05. Panel D: A clonogenic assay was used to examine surviving fraction in 2 Gy irradiated Cal27 cells pretreated with and without G6PD inhibitor, DHEA. Representative dishes of colonies are included for comparison. Asterisks represent statistical significance of combined treatment compared to radiation or DHEA treatment alone. n = 3; P < 0.05.