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. 2014 Nov 5;9(11):e111888. doi: 10.1371/journal.pone.0111888

Figure 2. In vivo NETs in hepatic sinusoids of the liver.

Figure 2

The hepatic sinusoids were observed at 24 h after LPS (20 mg/kg) intraperitoneal administration (n = 10). NETs (red) were detected by SYTOX Orange, Alexa Fluor 594-labeled anti-histone antibody, or Alexa Fluor 594-labeled anti-NE antibody, respectively. NETs were characterized as spot-like structures anchored to leukocytes (A-i, ii; Movie S3), cell-free DNA fragments (A-iii; Movie S4), and cell-free DNA fragments within platelet aggregates (A-iv). Isolectin GS-IB4 stained hepatic sinusoidal endothelial cells were observed in LPS-treated mice (A-v) and normal mice (A-vi). The number of NETs per FOV and the score of endothelial integrity were determined as described in materials and methods, respectively. The number of NETs per FOV (B) was significantly greater in LPS treated mice than normal control mice (1.7±0.2 vs 0.2±0.1). The score of endothelial integrity (C) was significantly less in LPS treated mice than control mice (.0±0.3 vs 3.6±0.2). Data was presented as mean+standard error. **P<0.01 versus control.