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Quantitative detection of PRRSV using a SYBR® Green qPCR assay. (A) Amplification of serially diluted PRRSV RNA (duplicates) containing 1 × 103 to 1 × 10−4 TCID50/ml. Amplification plots are shown from 1 × 103 to 1 × 10−2 TCID50/ml. (B) Standard curve for quantification of the partial ORF7 gene of PRRSV. The standard curve plots Ct values against the log of 10-fold dilutions of viral RNA equivalent to 1 × 103 to 1 × 10−4 TCID50/ml.
