Table 1. Comparison of the real-time PCR assay with the nested PCR assay for detection of type 1 PRRSV from serum samples collected from pigs living at the outbreak farm.
| Age (Days old) |
January 2008 | September 2008 | October 2009 | January 2011 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Real-time PCR (%) |
Nested PCR ((%) |
Real-time PCR ((%) |
Nested PCR ((%) |
Real-time PCR ((%) |
Nested PCR ((%) |
Real-time PCR ((%) |
Nested PCR ((%) |
|||||||||
| + | – | + | – | + | – | + | – | + | – | + | – | + | – | + | – | |
| < 60 | 0 | 10 (100) | 0 | 10 (100) | 9 (90) | 1 (10) | 6 (60) | 4 (40) | 0 | 10 (100) | 0 | 10 (100) | 0 | 5 (100) | 0 | 5 (100) |
| 60 < 120 | 0 | 10 (100) | 0 | 10 (100) | 8 (80) | 2 (20) | 6 (60) | 4 (40) | 5 (50) | 5 (50) | 4 (40) | 6 (60) | 0 | 10 (100) | 0 | 10 (100) |
| 120 < | 0 | 10 (100) | 0 | 10 (100) | 2 (20) | 8 (80) | 1 (10) | 9 (90) | 0 | 10 (100) | 0 | 10 (100) | 0 | 5 (100) | 0 | 5 (100) |
| Sow | 0 | 5 (100) | 0 | 5 (100) | 0 | 5 (100) | 0 | 5 (100) | 0 | 10 (100) | 0 | 5 (100) | 0 | 5 (100) | 0 | 5 (100) |
| Total | 0 (0) | 35 (100) | 0 (0) | 35 (100) | 19 (54.3) | 16 (45.7) | 13 (37) | 22 (63) | 5 (14.2) | 30 (85.7) | 4 (11) | 31 (89) | 0 (0) | 25 (100) | 0 (0) | 25 (100) |