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. Author manuscript; available in PMC: 2015 Jul 31.
Published in final edited form as: Cell. 2014 Jul 31;158(3):579–592. doi: 10.1016/j.cell.2014.05.051

Figure 7. Progenitor cells can differentiate to HCC or CC in vivo.

Figure 7

(A) Schematic of transposon vector combination used to generate HCCs and representative H/E stainings of tumors after 6 weeks. Arrows indicate small progenitor tumor cells. Bar= 100 μm (B) GFP (Yap;shp53), dsRED (shAPC), HNF4a, and nestin staining of progenitor like tumor cells and HCC-like tumor cells. Bars indicate 200 μm (C) Immunofluorescence images of a tumor nodule of Yap/shp53/shAPC injected mice. GFP indicates Yap/shp53, dsRED indicates shAPC, HNF4a was detected by Alexa-648 secondary antibody. DAPI was used for counterstaining. Bars reflect 100 μm (D) Schematic of transposon vectors used to generate CC and representative HE sections of liver tumors 6 weeks after injection. (E) GFP (Yap;shp53), mCherry (NICD), HNF1b, and nestin immunohistochemistry of cholangiocarcinomas. Bars indicate 100 μm.